Contributed by Long-Xi Yu and Mark Sorrells The co-dominant marker, BF145935 derived from a wheat EST (Ayala-Navarrete et al. 2007) and the dominant marker, Gb were used for haplotyping Sr25 (Yu et al., 2010). Liu et al. (2009) validated marker BF145935 using 42 wheat lines and indicated that two DNA fragments amplified in most lines. The upper band of Wheatear was smaller than that of non-Sr25 lines. This DNA fragment is located on the 7Ae#1 segment that was translocated onto wheat chromosome 7DL (Ayala-Navarrete et al. 2007; Liu et al., 2009). ... |
Contributed by Long-Xi Yu and Mark Sorrells Sr22 was previously mapped on the long arm of chromosome 7A (Khan et al., 2005). Three linked markers, cfa2019, cfa2123 and BARC121 (Miranda et al., 2007), were used for haplotyping this locus in our study (Yu et al. 2010). ... |
| Mark Sorrells contributed an excel spread sheet that includes resistance genes, molecular markers for those genes, and the corresponding pcr primers. ... |